{"id":20119,"date":"2024-05-25T23:27:53","date_gmt":"2024-05-25T21:27:53","guid":{"rendered":"https:\/\/looketmedecine.fr\/?p=20119"},"modified":"2024-10-05T23:44:45","modified_gmt":"2024-10-05T21:44:45","slug":"en-plaquettes-prp-comme-alternative-au-serum-de-veau-foetal-fbs-dans-lexpansion-des-myoblastes-humains-un-protocole-in-vitro","status":"publish","type":"post","link":"https:\/\/looketmedecine.fr\/en\/en-plaquettes-prp-comme-alternative-au-serum-de-veau-foetal-fbs-dans-lexpansion-des-myoblastes-humains-un-protocole-in-vitro\/","title":{"rendered":"LM N\u00b01\/ 2024. PLATELET-RICH PLASMA (PRP) AS AN ALTERNATIVE TO FETAL CALF SERUM (FBS) IN THE EXPANSION OF HUMAN MYOBLASTS: AN IN VITRO PROTOCOL.                                                                                               Axel TOLLANCE, Diego MICHEL, Alexandre PROLA, Axelle BOUCHE, Antoine TURZI, Didier HANNOUCHE, Thomas LAUMONIER and Sarah BERNDT"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"20119\" class=\"elementor elementor-20119\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3f51270 e-con-full e-flex e-con e-parent\" data-id=\"3f51270\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6ce2f93 elementor-widget elementor-widget-heading\" data-id=\"6ce2f93\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"> \u00c9VALUATION DU PLASMA RICHE EN PLAQUETTES (PRP) COMME ALTERNATIVE AU S\u00c9RUM DE VEAU F\u0152TAL (FBS) DANS L\u2019EXPANSION DES MYOBLASTES HUMAINS :\nUN PROTOCOLE IN VITRO.\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fe0cba7 elementor-widget elementor-widget-heading\" data-id=\"fe0cba7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Axel TOLLANCE1,3, Diego MICHEL1, Alexandre PROLA2, Axelle BOUCHE1,2, Antoine TURZI3, Didier HANNOUCHE1,2, Thomas LAUMONIER1,2 and Sarah BERNDT3<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d41d9e8 elementor-widget elementor-widget-text-editor\" data-id=\"d41d9e8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul>\n<li><strong>1\u00a0<em>Department<\/em> <em>of<\/em> <em>Orthopedic<\/em> <em>Surgery,<\/em> <em>Geneva<\/em> <em>University<\/em> <em>Hospitals<\/em> <em>&amp;<\/em> <em>Faculty<\/em> <em>of<\/em> <em>Medicine,<\/em> <em>Geneva, <\/em><em>Switzerland.<\/em><\/strong><\/li>\n<li><strong>2 <em>Department of Cell Physiology and Metabolism, Faculty of Medicine, Geneva, Switzerland.<\/em><\/strong><\/li>\n<li><strong>3 <em>Regen Lab SA, 1052 Le Mont-sur-Lausanne, Switzerland.<\/em><\/strong><\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d7119d0 e-flex e-con-boxed e-con e-parent\" data-id=\"d7119d0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-261776e elementor-widget elementor-widget-text-editor\" data-id=\"261776e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Mots-cl\u00e9s : plasma riche en plaquettes, cellules souches musculaires, expansion cellulaire, r\u00e9g\u00e9n\u00e9ration musculaire.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4248e7e elementor-widget elementor-widget-text-editor\" data-id=\"4248e7e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>INTRODUCTION<\/strong><\/h4>\n<p><strong>Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent un d\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent une morbidit\u00e9 significative. <\/strong><\/p>\n<p><strong>La r\u00e9g\u00e9n\u00e9ration musculaire est un processus complexe qui implique la participation de diverses populations cellulaires, notamment les cellules souches musculaires (CSM), qui jouent un r\u00f4le central dans la restauration de l\u2019int\u00e9grit\u00e9 et de la fonction musculaires apr\u00e8s des l\u00e9sions [<a href=\"#_bookmark0\">1<\/a>, <a href=\"#_bookmark1\">2<\/a>]. <\/strong><\/p>\n<p><strong>Cependant, dans des conditions pathologiques telles que la dystrophie musculaire de Duchenne (DMD), les capacit\u00e9s de r\u00e9g\u00e9n\u00e9ration musculaire sont compromises, mettant en \u00e9vidence la n\u00e9cessit\u00e9 de d\u00e9velopper de nouvelles approches th\u00e9rapeutiques pour traiter ces maladies musculaires d\u00e9bilitantes.<\/strong><\/p>\n<h4>\u00a0<\/h4>\n<h4><strong>LES CELLULES SOUCHES MUSCULAIRES ET LEUR R\u00d4LE DANS LA R\u00c9G\u00c9N\u00c9RATION MUSCULAIRE<\/strong><\/h4>\n<p><strong>Les CSM r\u00e9sident dans un \u00e9tat quiescent entre la fibre musculaire et la lame basale, pr\u00eates \u00e0 \u00eatre activ\u00e9es en cas de besoin, comme lors d\u2019une l\u00e9sion musculaire. <\/strong><\/p>\n<p><strong>Ces cellules jouent un r\u00f4le crucial dans la r\u00e9g\u00e9n\u00e9ration et la croissance musculaire postnatale, assurant ainsi l\u2019int\u00e9grit\u00e9 et la fonctionnalit\u00e9 du tissu musculaire tout au long de la vie. Lorsque des dommages surviennent dans le muscle, que ce soit suite \u00e0 un traumatisme ou \u00e0 une maladie, les CSM sont activ\u00e9es pour r\u00e9pondre \u00e0 la demande de r\u00e9paration. <\/strong><\/p>\n<p><strong>Ce processus implique une activation des CSM suivie d\u2019une prolif\u00e9ration des cellules prog\u00e9nitrices, appel\u00e9es myoblastes, qui peuvent ensuite se diff\u00e9rencier en cellules musculaires matures et int\u00e9grer le tissu musculaire pour restaurer sa fonctionnalit\u00e9 [<a href=\"#_bookmark2\">3<\/a>].<\/strong><\/p>\n<p><strong>Dans des conditions pathologiques telles que la DMD, ces processus de r\u00e9g\u00e9n\u00e9ration sont compromis en raison de dysfonctionnements des CSM. <\/strong><\/p>\n<p><strong>Dans le cas de la DMD, une mutation dans le g\u00e8ne de la dystrophine entra\u00eene une d\u00e9g\u00e9n\u00e9rescence musculaire progressive, conduisant \u00e0 une diminution de la fonction musculaire et \u00e0 une incapacit\u00e9 \u00e0 r\u00e9g\u00e9n\u00e9rer efficacement les tissus musculaires endommag\u00e9s. <\/strong><\/p>\n<p><strong>Les CSM dans les muscles affect\u00e9s par la DMD montrent une alt\u00e9ration de leur capacit\u00e9 prolif\u00e9rative et une diminution de leur potentiel de diff\u00e9renciation, ce qui contribue \u00e0 la progression de la maladie et \u00e0 la d\u00e9t\u00e9rioration musculaire.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee83f5b elementor-widget elementor-widget-text-editor\" data-id=\"ee83f5b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>TH\u00c9RAPIES BAS\u00c9ESSUR LES CELLULES SOUCHES MUSCULAIRES<\/strong><\/h4>\n<p><strong>Les th\u00e9rapies bas\u00e9es sur les CSM ont suscit\u00e9 un int\u00e9r\u00eat consid\u00e9rable en tant que strat\u00e9gies potentielles pour la r\u00e9paration musculaire. Les l\u00e9sions musculaires traumatiques, telles que les ruptures ou les d\u00e9chirures musculaires, pourraient b\u00e9n\u00e9ficier de th\u00e9rapies bas\u00e9es sur les cellules souches musculaires pour acc\u00e9l\u00e9rer le processus de gu\u00e9rison et restaurer la fonction musculaire normale [<a href=\"#_bookmark3\">4<\/a>, <a href=\"#_bookmark4\">5<\/a>]. En injectant des cellules souches musculaires directement dans la zone l\u00e9s\u00e9e, il est possible de favoriser la r\u00e9g\u00e9n\u00e9ration tissulaire et de r\u00e9duire la formation de cicatrices, ce qui pourrait am\u00e9liorer les r\u00e9sultats cliniques pour les patients souffrant de telles blessures.<\/strong><\/p>\n<p><strong>Cependant, malgr\u00e9 leur potentiel prometteur, plusieurs d\u00e9fis restent \u00e0 relever avant que les th\u00e9rapies bas\u00e9es sur les cellules souches musculaires ne deviennent courantes en clinique. <\/strong><\/p>\n<p><strong>Ces d\u00e9fis comprennent la n\u00e9cessit\u00e9 de d\u00e9velopper des m\u00e9thodes efficaces pour isoler et cultiver les cellules souches musculaires en quantit\u00e9s suffisantes, ainsi que la compr\u00e9hension des m\u00e9canismes sous-jacents de leur diff\u00e9renciation et de leur int\u00e9gration dans les tissus musculaires endommag\u00e9s. <\/strong><\/p>\n<p><strong>Une pr\u00e9occupation majeure r\u00e9side dans la s\u00e9lection et l\u2019expansion des cellules appropri\u00e9es en quantit\u00e9s suffisantes pour une application th\u00e9rapeutique efficace [<a href=\"#_bookmark5\">6<\/a>].<\/strong><\/p>\n<p><strong>\u00c0 cet \u00e9gard, l\u2019identification de nouvelles sources de cellules, telles que les cellules r\u00e9serve musculaire humaine (CRMH), repr\u00e9sente un progr\u00e8s significatif. <\/strong><\/p>\n<p><strong>Ces cellules partagent de nombreuses caract\u00e9ristiques des CSM quiescentes et pr\u00e9sentent un potentiel prometteur pour la th\u00e9rapie cellulaire musculaire<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7bb6f07 elementor-widget elementor-widget-text-editor\" data-id=\"7bb6f07\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>EXPLICATION DU MOD\u00c8LE <em>IN<\/em> <em>VITRO<\/em> UTILIS\u00c9<\/strong><\/h4>\n<p><strong>Pour mieux comprendre le comportement des cellules musculaires et \u00e9valuer l\u2019efficacit\u00e9 de nouvelles th\u00e9rapies, nous avons utilis\u00e9 un mod\u00e8le <em>in vitro <\/em>bas\u00e9 sur des cellules souches humaines primaires. <\/strong><\/p>\n<p><strong>Nous avons d\u2019abord extrait les cellules mononucl\u00e9es d\u2019une biopsie musculaire, avons sp\u00e9cifiquement s\u00e9lectionn\u00e9es les cellules souches musculaire par cytom\u00e9trie de flux, puis les avons laiss\u00e9es prolif\u00e9rer en tant que myoblastes. <\/strong><\/p>\n<p><strong>Une fois confluents, les myoblastes peuvent \u00eatre diff\u00e9renci\u00e9s dans un milieu pauvre en facteur de croissance. Environ 70 % des myoblastes fusionnent pour former de grandes cellules polynucl\u00e9\u00e9es appel\u00e9es myotubes, tandis que les cellules restantes restent mononucl\u00e9\u00e9es et acqui\u00e8rent des caract\u00e9ristiques de cellules souches. <\/strong><\/p>\n<p><strong>Ces cellules sont appel\u00e9es CMRH et sont le pendant <em>in vitro <\/em>des cellules souches. <\/strong><\/p>\n<p><strong>Elles partagent de nombreuses caract\u00e9ristiques des CSM, comme par exemple leur \u00e9tat de quiescence, l\u2019expression du facteur de transcription Pax7, ou encore leur capacit\u00e9 \u00e0 \u00eatre activ\u00e9es apr\u00e8s stimulation [<a href=\"#_bookmark6\">7<\/a>].<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a31700b elementor-widget elementor-widget-image\" data-id=\"a31700b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1494\" height=\"786\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39.png\" class=\"attachment-full size-full wp-image-21196\" alt=\"\" srcset=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39.png 1494w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39-300x158.png 300w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39-1024x539.png 1024w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39-768x404.png 768w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.20.39-18x9.png 18w\" sizes=\"(max-width: 1494px) 100vw, 1494px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-eb7ac15 elementor-widget elementor-widget-image\" data-id=\"eb7ac15\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1466\" height=\"602\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31.png\" class=\"attachment-full size-full wp-image-21200\" alt=\"\" srcset=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31.png 1466w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31-300x123.png 300w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31-1024x420.png 1024w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31-768x315.png 768w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/Capture-decran-2024-06-08-a-09.21.31-18x7.png 18w\" sizes=\"(max-width: 1466px) 100vw, 1466px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e3bc95d elementor-widget elementor-widget-text-editor\" data-id=\"e3bc95d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #800080;\"><strong><em>Figure 1. <\/em><\/strong><strong>A. <\/strong><em>Repr\u00e9sentation sch\u00e9matique du processus de r\u00e9g\u00e9n\u00e9ration in vivo. <\/em><strong>(1) <\/strong><em>Suite \u00e0 une l\u00e9sion, des signaux adapt\u00e9s activent les cellules souches musculaires (CSM) et induisent leur migration vers le site de la l\u00e9sion. Ces CSM activ\u00e9es r\u00e9-entrent dans le cycle cellulaire et sont appel\u00e9es myoblastes. <\/em><strong>(2) <\/strong><em>Les myoblastes sur le site de la l\u00e9sion prolif\u00e8rent jusqu\u2019\u00e0 atteindre un nombre appropri\u00e9 permettant la restauration du muscle endommag\u00e9. <\/em><strong>(3) <\/strong><em>Les myoblastes se diff\u00e9rencient, arr\u00eatent leur cycle cellulaire et entrent en phase post-mitotique. <\/em><strong>(4) <\/strong><em>Finalement, ils fusionnent entre eux ou avec la fibre endommag\u00e9e pour restaurer l\u2019int\u00e9gralit\u00e9 du muscle. <\/em><strong>(5) <\/strong><em>En parall\u00e8le, pendant l\u2019activation\/prolif\u00e9ration des myoblastes, une sous-population reste quiescente et reconstitue le stock de CSM pour de futures r\u00e9g\u00e9n\u00e9rations.<\/em><\/span><\/p>\n<p><span style=\"color: #800080;\"><strong>B. <\/strong><em>Repr\u00e9sentation sch\u00e9matique de notre mod\u00e8le <\/em>in vitro <em>et photo repr\u00e9sentative d\u2019une culture de myotubes et MuRC apr\u00e8s 48 heures de diff\u00e9renciation<\/em><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7c75356 elementor-widget elementor-widget-text-editor\" data-id=\"7c75356\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>ALTERNATIVES AU S\u00c9RUM DE VEAU F\u0152TAL : LE PLASMA RICHE EN PLAQUETTES (PRP)<\/strong><\/h4>\n<p><strong>Une autre consid\u00e9ration importante concerne les conditions de culture cellulaire utilis\u00e9es pour l\u2019expansion des cellules musculaires en laboratoire. <\/strong><\/p>\n<p><strong>Traditionnellement, le s\u00e9rum de b\u0153uf f\u0153tal (FBS) a \u00e9t\u00e9 largement utilis\u00e9 comme source de nutriments et de facteurs de croissance pour la culture cellulaire. <\/strong><\/p>\n<p><strong>Cependant, l\u2019utilisation du FBS pr\u00e9sente des inconv\u00e9nients, notamment des risques de contamination, d\u2019immunog\u00e9nicit\u00e9 et des pr\u00e9occupations \u00e9thiques li\u00e9es \u00e0 son origine animale [<a href=\"#_bookmark7\">8<\/a>, <a href=\"#_bookmark8\">9<\/a>, <a href=\"#_bookmark9\">10<\/a>, <a href=\"#_bookmark10\">11<\/a>].<\/strong><\/p>\n<p><strong>Pour pallier ces probl\u00e8mes, des alternatives au FBS sont activement recherch\u00e9es. Le plasma riche en plaquettes (PRP) est l\u2019une de ces alternatives potentielles. <\/strong><\/p>\n<p><strong>Le PRP est un produit biologique d\u00e9riv\u00e9 du sang, riche en facteurs de croissance contenus dans les granules alpha des plaquettes. <\/strong><\/p>\n<p><strong>Lorsqu\u2019il est activ\u00e9, le PRP lib\u00e8re ces facteurs de croissance, favorisant ainsi la r\u00e9g\u00e9n\u00e9ration tissulaire. <\/strong><\/p>\n<p><strong>Les diff\u00e9rentes pr\u00e9parations de PRP comprennent le PRP riche en leucocytes (LR-PRP) et le PRP pauvre en leucocytes (LP-PRP), ainsi que le lysat plaquettaire qui sont des d\u00e9riv\u00e9s du PRP [<a href=\"#_bookmark11\">12<\/a>, <a href=\"#_bookmark12\">13<\/a>, <a href=\"#_bookmark13\">14<\/a>, <a href=\"#_bookmark14\">15<\/a>, <a href=\"#_bookmark15\">16<\/a>].<\/strong><\/p>\n<p><strong>Des \u00e9tudes pr\u00e9liminaires ont montr\u00e9 que le PRP, qu\u2019il soit d\u2019origine autologue ou allog\u00e9nique, peut favoriser la prolif\u00e9ration de cellules <em>in vitro<\/em>. <\/strong><\/p>\n<p><strong>Ces effets b\u00e9n\u00e9fiques sont attribu\u00e9s aux facteurs de croissance contenus dans le PRP, tels que le facteur de croissance d\u00e9riv\u00e9 des plaquettes (PDGF), le facteur de croissance transformant b\u00eata (TGF-\u03b2) et le facteur de croissance endoth\u00e9lial vasculaire (VEGF) [<a href=\"#_bookmark16\">17<\/a>, <a href=\"#_bookmark17\">18<\/a>, <a href=\"#_bookmark18\">19<\/a>, <a href=\"#_bookmark19\">20<\/a>, <a href=\"#_bookmark20\">21<\/a>]. <\/strong><\/p>\n<p><strong>Cependant, des recherches suppl\u00e9mentaires sont n\u00e9cessaires pour \u00e9valuer pleinement leur efficacit\u00e9 et leur s\u00e9curit\u00e9, ainsi que pour r\u00e9soudre les d\u00e9fis pratiques associ\u00e9s \u00e0 leur utilisation \u00e0 grande \u00e9chelle dans un contexte clinique.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4b455e6 elementor-widget elementor-widget-text-editor\" data-id=\"4b455e6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>COMBINER LE PRP AVEC DE L\u2019ACIDE HYALURONIQUE<\/strong><\/h4>\n<p><strong>L\u2019acide hyaluronique (HA) est un composant essentiel de la matrice extracellulaire qui joue un r\u00f4le crucial dans divers processus biologiques, y compris la culture de cellules <em>in vitro<\/em>. <\/strong><\/p>\n<p><strong>Son utilisation pr\u00e9sente plusieurs avantages significatifs dans les cultures cellulaires, offrant un environnement propice \u00e0 la croissance, \u00e0 la diff\u00e9renciation et \u00e0 la fonctionnalit\u00e9 des cellules [<a href=\"#_bookmark21\">22<\/a>].<\/strong><\/p>\n<p><strong>L\u2019un des principaux avantages de l\u2019utilisation de l\u2019acide hyaluronique dans les cultures de cellules <em>in vitro <\/em>est sa capacit\u00e9 \u00e0 stimuler la prolif\u00e9ration cellulaire. <\/strong><\/p>\n<p><strong>En fournissant un substrat tridimensionnel et une structure de soutien, l\u2019acide hyaluronique cr\u00e9e un microenvironnement favorable \u00e0 la croissance cellulaire, ce qui peut augmenter le taux de prolif\u00e9ration des cellules en culture [<a href=\"#_bookmark22\">23<\/a>].<\/strong><\/p>\n<p><strong>De plus, l\u2019acide hyaluronique aide \u00e0 maintenir la viabilit\u00e9 des cellules en fournissant une hydratation ad\u00e9quate et en r\u00e9gulant l\u2019\u00e9quilibre osmotique dans les cultures cellulaires. <\/strong><\/p>\n<p><strong>Il agit comme un r\u00e9servoir d\u2019eau, retenant l\u2019humidit\u00e9 n\u00e9cessaire pour maintenir les cellules en bonne sant\u00e9 et fonctionnelles tout au long de la culture <em>in vitro<\/em>.<\/strong><\/p>\n<p><strong>Un autre avantage majeur de l\u2019utilisation de l\u2019acide hyaluronique est sa capacit\u00e9 \u00e0 am\u00e9liorer l\u2019adh\u00e9sion cellulaire. <\/strong><\/p>\n<p><strong>En fournissant des sites de liaison pour les r\u00e9cepteurs cellulaires sp\u00e9cifiques, il favorise l\u2019attachement des cellules \u00e0 la surface de culture, ce qui est essentiel pour leur survie, leur prolif\u00e9ration et leur diff\u00e9renciation appropri\u00e9es [<a href=\"#_bookmark23\">24<\/a>].<\/strong><\/p>\n<p><strong>Enfin, l\u2019acide hyaluronique peut jouer un r\u00f4le dans la modulation de la diff\u00e9renciation cellulaire en influen\u00e7ant les interactions cellule-matrice et la signalisation cellulaire. <\/strong><\/p>\n<p><strong>Des \u00e9tudes ont montr\u00e9 que sa pr\u00e9sence dans les cultures cellulaires peut favoriser la diff\u00e9renciation cellulaire dans certains types cellulaires, tels que les cellules souches m\u00e9senchymateuses, les chondrocytes et les adipocytes.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-feda4d5 elementor-widget elementor-widget-text-editor\" data-id=\"feda4d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>\u00c9TUDES FUTURES : \u00c9VALUATION DE L\u2019EFFET DU PRP ET DU PRP-HA<\/strong><\/h4>\n<p><strong>Ainsi, dans le cadre de cette \u00e9tude, nous pr\u00e9voyons d\u2019investiguer deux d\u00e9riv\u00e9s du sang humain (PRP humain allog\u00e9nique non activ\u00e9 [PRP] ou PRP humain allog\u00e9nique non activ\u00e9 combin\u00e9 \u00e0 l\u2019acide hyaluronique [PRP-HA]), utilis\u00e9s comme substituts de FBS pour l\u2019expansion <em>in vitro <\/em>de myoblastes humains primaires avant leur diff\u00e9renciation myog\u00e9nique. <\/strong><\/p>\n<p><strong>Nous \u00e9valuerons le taux de prolif\u00e9ration <em>in vitro<\/em>, l\u2019expression de diff\u00e9rents marqueurs de surface cellulaire, leur capacit\u00e9 de diff\u00e9renciation myog\u00e9nique et le niveau d\u2019expression du facteur de transcription Pax7 dans les cellules r\u00e9serves musculaires humaines g\u00e9n\u00e9r\u00e9es <em>in vitro<\/em>. <\/strong><\/p>\n<p><strong>Nous voulons tester si le PRP ou le PRP-HA peuvent efficacement remplacer le FBS x\u00e9nog\u00e9nique dans l\u2019expansion <em>in vitro <\/em>de myoblastes humains, et si ces conditions de culture n\u2019alt\u00e8rent pas leur ph\u00e9notype inflammatoire. <\/strong><\/p>\n<p><strong>De plus, nous allons tester si les myoblastes humains, cultiv\u00e9s dans un milieu de croissance suppl\u00e9ment\u00e9 au PRP ou au PRP-HA, g\u00e9n\u00e8rent un pourcentage plus \u00e9lev\u00e9 de cellules r\u00e9serves Pax7High par rapport aux myoblastes trait\u00e9s au FBS.<\/strong><\/p>\n<p><span style=\"color: #800080;\"><strong>Nos r\u00e9sultats pr\u00e9liminaires sugg\u00e8rent fortement que le PRP humain allog\u00e9nique non activ\u00e9 ou le PRP-HA sont des alternatives efficaces et appropri\u00e9es au FBS pour la g\u00e9n\u00e9ration de cellules r\u00e9serves musculaires humaines dans un \u00e9tat de quiescence plus profonde.<\/strong><\/span><\/p>\n<p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3c684d elementor-widget elementor-widget-text-editor\" data-id=\"d3c684d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #339966;\"><strong>R\u00c9F\u00c9RENCES<\/strong><\/span><\/p>\n<ol>\n<li><span style=\"color: #339966;\">Relaix, P.S. Zammit, Satellite cells are essential for skeletal muscle regeneration: the cell on the edge returns centre stage, Development 139 (2012) 2845-56. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1242\/dev.069088\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1242\/dev.069088<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">A. Collins, I. Olsen, P.S. Zammit, L. Heslop, A. Petrie, T.A. Partridge, J.E. Morgan, Stem Cell Function, Self-Renewal, and Behavioral Heterogeneity of Cells from the Adult Muscle Satellite Cell Niche, Cell 122 (2005) 289-301. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.cell.2005.05.010\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.cell.2005.05.010\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">org\/10.1016\/j.cell.2005.05.010<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Fu, H. Wang, P. Hu, Stem cell activation in skeletal muscle regeneration, Cell. Mol. Life Sci. 72 (2015) 1663-77. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1007\/s00018-014-1819-5\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1007\/s00018-014-1819-5\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">doi.org\/10.1007\/s00018-014-1819-5<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Sun, C. Serra, G. Lee, K.R. Wagner, Stem cell-based therapies for Duchenne muscular dystrophy, Experimental Neurology 323 (2020) 113086. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.expneurol.2019.113086\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.expneurol.2019.113086<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">N. Judson, F.M.V. Rossi, Towards stem cell therapies for skeletal muscle repair, Npj Regen Med 5 (2020) 10. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1038\/s41536-020-0094-3\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1038\/s41536-020-0094-3\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">doi.org\/10.1038\/s41536-020-0094-3<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Lorant, C. Saury, C. Schleder, F. Robriquet, B. Lieubeau, E. N\u00e9groni, I. Leroux, L. Chabrand, S. Viau, C. Babarit, M. Ledevin, L. Dubreil, A. Hamel, A. Magot, C. Thorin, L. Guevel, B. Delorme, Y. P\u00e9r\u00e9on, G. Butler-Browne, V. Mouly, K. Rouger, Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle, Molecular Therapy 26 (2018) 618-33. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.ymthe.2017.10.013\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.ymthe.2017.10.013<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Bouche, B. Borner, C. Richard, Y. Grand, D. Hannouche, T. Laumonier, In vitro-generated human muscle reserve cells are heterogeneous for Pax7 with distinct molecular states and metabolic profiles, Stem Cell Res Ther 14 (2023) 243. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1186\/s13287-023-03483-5\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1186\/s13287-023-03483-5<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">E.A. Jochems, J.B.F. Van Der Valk, F.R. Stafleu, V. Baumans, The Use of Fetal Bovine Serum: Ethical or Scientific Problem?, Altern Lab Anim 30 (2002) 219-27. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1177\/026119290203000208\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1177\/026119290203000208<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Tekkatte, G.P. Gunasingh, K.M. Cherian, K. Sankaranarayanan, \u201cHumanized\u201d Stem Cell Culture Techniques: The Animal Serum Controversy, Stem Cells International 2011 (2011) 1-14. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.4061\/2011\/504723\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.4061\/2011\/504723<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">L. Spees, C.A. Gregory, H. Singh, H.A. Tucker, A. Peister, P.J. Lynch, S.-C. Hsu, J. Smith, D.J. Prockop, Internalized Antigens Must Be Removed to Prepare Hypoimmunogenic Mesenchymal Stem Cells for Cell and Gene Therapy, Molecular Therapy 9 (2004) 747-56. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.ymthe.2004.02.012\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.ymthe.2004.02.012<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Karnieli, O.M. Friedner, J.G. Allickson, N. Zhang, S. Jung, D. Fiorentini, E. Abraham, S.S. Eaker, T.K. Yong, A. Chan,<\/span><\/li>\n<li><span style=\"color: #339966;\">Griffiths, A.K. Wehn, S. Oh, O. Karnieli, A consensus introduction to serum replacements and serum-free media for cellular therapies, Cytotherapy 19 (2017) 155-69. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.jcyt.2016.11.011\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.jcyt.2016.11.011<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">R. Kark, J.M. Karp, J.E. Davies, Platelet releasate increases the proliferation and migration of bone marrow\u2010derived cells cultured under osteogenic conditions, Clinical Oral Implants Res 17 (2006) 321-7. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1111\/j.1600-0501.2005.01189.x\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1111\/j.1600-0501.2005.01189.x\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">org\/10.1111\/j.1600-0501.2005.01189.x<\/a>.<\/span><\/li>\n<\/ol>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\n<ol start=\"13\">\n<li><span style=\"color: #339966;\">Scully, P. Sfyri, S. Verpoorten, P. Papadopoulos, M.C. Mu\u00f1oz\u2010Turrillas, R. Mitchell, A. Aburima, K. Patel, L. Guti\u00e9rrez,<\/span><\/li>\n<\/ol>\n<p><span style=\"color: #339966;\">K.M. Naseem, A. Matsakas, Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury, Acta Physiol 225 (2019). <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1111\/apha.13207\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1111\/apha.13207\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">org\/10.1111\/apha.13207<\/a>.<\/span><\/p>\n<ol start=\"14\">\n<li><span style=\"color: #339966;\">Mautner, G.A. Malanga, J. Smith, B. Shiple, V. Ibrahim, S. Sampson, J.E. Bowen, A Call for a Standard Classification System for Future Biologic Research: The Rationale for New PRP Nomenclature, PM&amp;R 7 (2015) S53-9. h<a style=\"color: #339966;\" href=\"ttps:\/\/doi.org\/10.1016\/j.pmrj.2015.02.005\">ttps:\/\/doi.<\/a> <a style=\"color: #339966;\" href=\"ttps:\/\/doi.org\/10.1016\/j.pmrj.2015.02.005\">org\/10.1016\/j.pmrj.2015.02.005<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">M. DeLong, R.P. Russell, A.D. Mazzocca, Platelet-Rich Plasma: The PAW Classification System, Arthroscopy: The Journal of Arthroscopic &amp; Related Surgery 28 (2012) 998-1009. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.arthro.2012.04.148\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.arthro.2012.04.148<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Burnouf, D. Strunk, M.B.C. Koh, K. Schallmoser, Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation?, Biomaterials 76 (2016) 371-87. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.biomaterials.2015.10.065\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.biomaterials.2015.10.065<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Dessels, M. Potgieter, M.S. Pepper, Making the Switch: Alternatives to Fetal Bovine Serum for Adipose-Derived Stromal Cell Expansion, Front. Cell Dev. Biol. 4 (2016). <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.3389\/fcell.2016.00115\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.3389\/fcell.2016.00115<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Patrikoski, M. Juntunen, S. Boucher, A. Campbell, M.C. Vemuri, B. Mannerstr\u00f6m, S. Miettinen, Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells, Stem Cell Res Ther 4 (2013) 27. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1186\/scrt175\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1186\/scrt175<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Shahdadfar, K. Fr\u00f8nsdal, T. Haug, F.P. Reinholt, J.E. Brinchmann, In Vitro Expansion of Human Mesenchymal Stem Cells: Choice of Serum Is a Determinant of Cell Proliferation, Differentiation, Gene Expression, and Transcriptome Stability, STEM CELLS 23 (2005) 1357-66. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1634\/stemcells.2005-0094\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1634\/stemcells.2005-0094<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Lange, F. Cakiroglu, A.-N. Spiess, H. Cappallo-Obermann, J. Dierlamm, A.R. Zander, Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine, J. Cell. Physiol. 213 (2007) 18-26. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1002\/jcp.21081\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1002\/jcp.21081<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Levoux, A. Prola, P. Lafuste, M. Gervais, N. Chevallier, Z. Koumaiha, K. Kefi, L. Braud, A. Schmitt, A. Yacia, A. Schirmann, B. Hersant, M. Sid-Ahmed, S. Ben Larbi, K. Komrskova, J. Rohlena, F. Relaix, J. Neuzil, A.-M. Rodriguez, Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming, Cell Metabolism 33 (2021) 283-99.e9. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1016\/j.cmet.2020.12.006\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1016\/j.cmet.2020.12.006<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">A. Burdick, G.D. Prestwich, Hyaluronic Acid Hydrogels for Biomedical Applications, Advanced Materials 23 (2011) H41\u2013 H56. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.1002\/adma.201003963\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.1002\/adma.201003963<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Abatangelo, V. Vindigni, G. Avruscio, L. Pandis, P. Brun, Hyaluronic Acid: Redefining Its Role, Cells 9 (2020) 1743. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.3390\/cells9071743\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.3390\/cells9071743<\/a>.<\/span><\/li>\n<li><span style=\"color: #339966;\">Lierova, J. Kasparova, A. Filipova, J. Cizkova, L. Pekarova, L. Korecka, N. Mannova, Z. Bilkova, Z. Sinkorova, Hyaluronic Acid: Known for Almost a Century, but Still in Vogue, Pharmaceutics 14 (2022) 838. <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.3390\/pharmaceutics14040838\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">https:\/\/doi.org\/10.3390\/<\/a> <a style=\"color: #339966;\" href=\"https:\/\/doi.org\/10.3390\/pharmaceutics14040838\" target=\"_blank\" rel=\"noopener noreferrer nofollow\">pharmaceutics14040838<\/a>.<\/span><\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0d5933c elementor-widget elementor-widget-image\" data-id=\"0d5933c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1359\" height=\"1754\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007.jpg\" class=\"attachment-full size-full wp-image-20136\" alt=\"\" srcset=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007.jpg 1359w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007-232x300.jpg 232w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007-793x1024.jpg 793w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007-768x991.jpg 768w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007-1190x1536.jpg 1190w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/038_044_Tollance_v2-premier-pas_page-0007-9x12.jpg 9w\" sizes=\"(max-width: 1359px) 100vw, 1359px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent un<br \/>\nd\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent une<br \/>\nmorbidit\u00e9 significative. La r\u00e9g\u00e9n\u00e9ration musculaire est un processus complexe qui implique la<br \/>\nparticipation de diverses populations cellulaires, notamment les cellules souches musculaires (CSM),<br \/>\nqui jouent un r\u00f4le central dans la restauration de l\u2019int\u00e9grit\u00e9 et de la fonction musculaires apr\u00e8s des<br \/>\nl\u00e9sions [1, 2]. Cependant, dans des conditions pathologiques telles que la dystrophie musculaire de<br \/>\nDuchenne (DMD), les capacit\u00e9s de r\u00e9g\u00e9n\u00e9ration musculaire sont compromises, mettant en \u00e9vidence<br \/>\nla n\u00e9cessit\u00e9 de d\u00e9velopper de nouvelles approches th\u00e9rapeutiques pour traiter ces maladies<br \/>\nmusculaires d\u00e9bilitantes.<\/p>\n","protected":false},"author":4,"featured_media":20126,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[160],"tags":[342,343],"auteur":[451,420,450,452,449,453,423,448],"mots-cles":[],"class_list":["post-20119","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-p-as","tag-lm1-2024-premiers-pas","tag-lm1-2024-replay","auteur-alexandre-prola","auteur-antoine-turzi","auteur-axel-tollance","auteur-axelle-bouche","auteur-didier-hannouche","auteur-diego-michel","auteur-sarah-berndt","auteur-thomas-laumonier"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>LM N\u00b01\/ 2024. LE PLASMA RICHE EN PLAQUETTES (PRP) COMME ALTERNATIVE AU S\u00c9RUM DE VEAU F\u0152TAL (FBS) DANS L\u2019EXPANSION DES MYOBLASTES HUMAINS : UN PROTOCOLE IN VITRO.  \u00a0                                              Axel TOLLANCE, Diego MICHEL, Alexandre PROLA, Axelle BOUCHE, Antoine TURZI, Didier HANNOUCHE, Thomas LAUMONIER et Sarah BERNDT - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique<\/title>\n<meta name=\"description\" content=\"Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent und\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent unemorbidit\u00e9 significative. La r\u00e9g\u00e9n\u00e9ration musculaire est un processus complexe qui implique laparticipation de diverses populations cellulaires, notamment les cellules souches musculaires (CSM),qui jouent un r\u00f4le central dans la restauration de l\u2019int\u00e9grit\u00e9 et de la fonction musculaires apr\u00e8s desl\u00e9sions [1, 2]. Cependant, dans des conditions pathologiques telles que la dystrophie musculaire deDuchenne (DMD), les capacit\u00e9s de r\u00e9g\u00e9n\u00e9ration musculaire sont compromises, mettant en \u00e9videncela n\u00e9cessit\u00e9 de d\u00e9velopper de nouvelles approches th\u00e9rapeutiques pour traiter ces maladiesmusculaires d\u00e9bilitantes.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/20119\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"LM N\u00b01\/ 2024. LE PLASMA RICHE EN PLAQUETTES (PRP) COMME ALTERNATIVE AU S\u00c9RUM DE VEAU F\u0152TAL (FBS) DANS L\u2019EXPANSION DES MYOBLASTES HUMAINS : UN PROTOCOLE IN VITRO.  \u00a0                                              Axel TOLLANCE, Diego MICHEL, Alexandre PROLA, Axelle BOUCHE, Antoine TURZI, Didier HANNOUCHE, Thomas LAUMONIER et Sarah BERNDT - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\" \/>\n<meta property=\"og:description\" content=\"Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent und\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent unemorbidit\u00e9 significative. La r\u00e9g\u00e9n\u00e9ration musculaire est un processus complexe qui implique laparticipation de diverses populations cellulaires, notamment les cellules souches musculaires (CSM),qui jouent un r\u00f4le central dans la restauration de l\u2019int\u00e9grit\u00e9 et de la fonction musculaires apr\u00e8s desl\u00e9sions [1, 2]. Cependant, dans des conditions pathologiques telles que la dystrophie musculaire deDuchenne (DMD), les capacit\u00e9s de r\u00e9g\u00e9n\u00e9ration musculaire sont compromises, mettant en \u00e9videncela n\u00e9cessit\u00e9 de d\u00e9velopper de nouvelles approches th\u00e9rapeutiques pour traiter ces maladiesmusculaires d\u00e9bilitantes.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/looketmedecine.fr\/en\/en-plaquettes-prp-comme-alternative-au-serum-de-veau-foetal-fbs-dans-lexpansion-des-myoblastes-humains-un-protocole-in-vitro\/\" \/>\n<meta property=\"og:site_name\" content=\"Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/looketmedecine.magazine\/\" \/>\n<meta property=\"article:published_time\" content=\"2024-05-25T21:27:53+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-10-05T21:44:45+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2024\/05\/telechargement.jpeg\" \/>\n\t<meta property=\"og:image:width\" content=\"225\" \/>\n\t<meta property=\"og:image:height\" content=\"225\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"yvan tenekeu\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"yvan tenekeu\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"14 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/en-plaquettes-prp-comme-alternative-au-serum-de-veau-foetal-fbs-dans-lexpansion-des-myoblastes-humains-un-protocole-in-vitro\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/en-plaquettes-prp-comme-alternative-au-serum-de-veau-foetal-fbs-dans-lexpansion-des-myoblastes-humains-un-protocole-in-vitro\\\/\"},\"author\":{\"name\":\"yvan tenekeu\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#\\\/schema\\\/person\\\/cff93d1c228784d84da238666226f645\"},\"headline\":\"LM N\u00b01\\\/ 2024. 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LE PLASMA RICHE EN PLAQUETTES (PRP) COMME ALTERNATIVE AU S\u00c9RUM DE VEAU F\u0152TAL (FBS) DANS L\u2019EXPANSION DES MYOBLASTES HUMAINS : UN PROTOCOLE IN VITRO.  \u00a0                                              Axel TOLLANCE, Diego MICHEL, Alexandre PROLA, Axelle BOUCHE, Antoine TURZI, Didier HANNOUCHE, Thomas LAUMONIER et Sarah BERNDT - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","description":"Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent und\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent unemorbidit\u00e9 significative. 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LE PLASMA RICHE EN PLAQUETTES (PRP) COMME ALTERNATIVE AU S\u00c9RUM DE VEAU F\u0152TAL (FBS) DANS L\u2019EXPANSION DES MYOBLASTES HUMAINS : UN PROTOCOLE IN VITRO.  \u00a0                                              Axel TOLLANCE, Diego MICHEL, Alexandre PROLA, Axelle BOUCHE, Antoine TURZI, Didier HANNOUCHE, Thomas LAUMONIER et Sarah BERNDT - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","og_description":"Les maladies musculaires, qu\u2019elles soient cong\u00e9nitales, acquises ou d\u00e9g\u00e9n\u00e9ratives, constituent und\u00e9fi majeur pour la sant\u00e9 publique, affectant la qualit\u00e9 de vie des patients et entra\u00eenant souvent unemorbidit\u00e9 significative. La r\u00e9g\u00e9n\u00e9ration musculaire est un processus complexe qui implique laparticipation de diverses populations cellulaires, notamment les cellules souches musculaires (CSM),qui jouent un r\u00f4le central dans la restauration de l\u2019int\u00e9grit\u00e9 et de la fonction musculaires apr\u00e8s desl\u00e9sions [1, 2]. 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