{"id":13537,"date":"2022-10-11T09:37:01","date_gmt":"2022-10-11T07:37:01","guid":{"rendered":"https:\/\/looketmedecine.fr\/?p=13537"},"modified":"2024-10-05T22:29:18","modified_gmt":"2024-10-05T20:29:18","slug":"lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques","status":"publish","type":"post","link":"https:\/\/looketmedecine.fr\/en\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/","title":{"rendered":"LM N\u00b0 2\/ 2022. Another look. Exosomes: an innovation in aesthetic medicine. Dr Sarah Berndt Doctor of Biomedical and Pharmaceutical Sciences"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"13537\" class=\"elementor elementor-13537\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e8fa00c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e8fa00c\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cab699f\" data-id=\"cab699f\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-15979e7 elementor-widget elementor-widget-text-editor\" data-id=\"15979e7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1 style=\"text-align: right;\"><span style=\"color: #0000ff;\"><strong>Un autre regard <\/strong><\/span><\/h1><h2 style=\"text-align: right;\">\u00a0<\/h2>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6c61303 elementor-widget elementor-widget-heading\" data-id=\"6c61303\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Les exosomes : une innovation en m\u00e9decine esth\u00e9tique\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-140c581 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"140c581\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0974032\" data-id=\"0974032\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bd5155a elementor-widget elementor-widget-heading\" data-id=\"bd5155a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Sarah Berndt, PhD\n\n\n \n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b74789 elementor-widget elementor-widget-image\" data-id=\"1b74789\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"282\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-300x282.png\" class=\"attachment-medium size-medium wp-image-13425\" alt=\"Sarah Berndt. PhD in Biomedical and Pharmaceutical Sciences- Head of Cell Therapy Research at Regen Lab SA\" srcset=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-300x282.png 300w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-1024x961.png 1024w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-768x721.png 768w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-1536x1442.png 1536w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31-13x12.png 13w, https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png 1736w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1500aa8 elementor-widget elementor-widget-text-editor\" data-id=\"1500aa8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><strong><span style=\"color: #ff0000;\">Docteur en sciences biom\u00e9dicales et pharmaceutiques<\/span><\/strong><br \/><strong><span style=\"color: #ff0000;\">Responsable de la recherche en th\u00e9rapie cellulaire chez Regen Lab SA (Suisse)<\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0e43bc9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0e43bc9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e8b3176\" data-id=\"e8b3176\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-811b886 elementor-widget elementor-widget-heading\" data-id=\"811b886\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Introduction<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c42451e elementor-widget elementor-widget-text-editor\" data-id=\"c42451e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong>Les exosomes sont de petites v\u00e9sicules extracellulaires rondes produites par presque tous les types de cellules et pr\u00e9sentes dans tous les fluides biologiques. Ils constituent un moyen de communication privil\u00e9gi\u00e9 entre les diff\u00e9rentes cellules de l&rsquo;organisme.\u00a0<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Chaque exosome peut contenir de nombreux constituants d&rsquo;une cellule comme des prot\u00e9ines cytosoliques et de surface cellulaire, des lipides, des ADN ou ARN et des m\u00e9tabolites.\u00a0 <\/span><span style=\"color: #000000;\">\u00c0 destination, l&rsquo;exosome fusionnera avec la membrane de la cellule cible et son contenu sera int\u00e9gr\u00e9.<\/span><\/p><p><span style=\"color: #000000;\">Les v\u00e9sicules sont tr\u00e8s sp\u00e9cifiques, puisque la nature de la cellule qui produit cet exosome influence ses propri\u00e9t\u00e9s physico-chimiques (Figure 1).\u00a0<\/span><\/p><p><span style=\"color: #0000ff;\"><strong>On pense que le r\u00f4le physiologique des exosomes est le maintien de l&rsquo;hom\u00e9ostasie cellulaire en r\u00e9gulant les constituants prot\u00e9iques exc\u00e9dentaires ou inutiles des cellules [1].<\/strong><\/span><\/p><p><span style=\"color: #800080;\">Les exosomes sont maintenant \u00e9valu\u00e9s comme <\/span><\/p><ul><li><span style=\"color: #800080;\">biomarqueurs de diff\u00e9rents \u00e9tats pathophysiologiques <\/span><\/li><li><span style=\"color: #800080;\">ou comme v\u00e9hicules potentiels pour l&rsquo;administration de m\u00e9dicaments [2] tels que <\/span><ul><li><span style=\"color: #800080;\">les ARN interf\u00e9rents courts, <\/span><\/li><li><span style=\"color: #800080;\">les oligonucl\u00e9otides antisens, <\/span><\/li><li><span style=\"color: #800080;\">les agents chimioth\u00e9rapeutiques <\/span><\/li><li><span style=\"color: #800080;\">et les modulateurs immunitaires<\/span><\/li><li><span style=\"color: #800080;\">avec le principal avantage d&rsquo;une administration cibl\u00e9e \u00e0 la cellule d\u00e9sir\u00e9e.<\/span><\/li><\/ul><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c54b27d elementor-widget elementor-widget-image\" data-id=\"c54b27d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-09.50.35.png\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cc6601d elementor-widget elementor-widget-text-editor\" data-id=\"cc6601d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"color: #0000ff;\"><strong>Figure 1 : Illustration sch\u00e9matique de la biogen\u00e8se et de la composition des exosomes.<\/strong>\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-769de06 elementor-widget elementor-widget-text-editor\" data-id=\"769de06\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em>Les exosomes et les microv\u00e9sicules sont des invaginations de la membrane plasmique qui font partie de la famille des v\u00e9sicules extracellulaires.\u00a0<br \/>La composition des exosomes comprend des lipides, des prot\u00e9ines cytosoliques, de l&rsquo;ADN, de l&rsquo;ARN et des prot\u00e9ines de la membrane de surface.\u00a0<br \/><\/em><\/p><p><em><span style=\"color: #0000ff;\"><strong>Les exosomes sont produits par diff\u00e9rents types de cellules et sont \u00e9tudi\u00e9s pour leur potentiel diagnostique et th\u00e9rapeutique.<\/strong><\/span><br \/><\/em><\/p><p><span style=\"color: #800080;\"><em>Parmi les principales fonctions physiologiques des exosomes, la recherche a mis en \u00e9vidence des r\u00f4les dans <\/em><\/span><\/p><ul><li><span style=\"color: #800080;\"><em>la survie cellulaire,<\/em><\/span><\/li><li><span style=\"color: #800080;\"><em> la prolif\u00e9ration, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>la migration, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>la diff\u00e9renciation,\u00a0<\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>la s\u00e9nescence, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>l&rsquo;immunomodulation, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>l&rsquo;angiogen\u00e8se, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>la cicatrisation, <\/em><\/span><\/li><li><span style=\"color: #800080;\"><em>la n\u00e9oplasie, entre autres [1].\u00a0<\/em><\/span><\/li><\/ul><p><em><span style=\"color: #0000ff;\">Compte tenu de leur diff\u00e9rence de taille, de la cellule d&rsquo;origine de la cargaison et de leur combinaison distincte, les exosomes sont connus pour \u00eatre tr\u00e8s h\u00e9t\u00e9rog\u00e8nes en termes de structure et de fonction [1]<\/span> <strong>(figure 2).<\/strong><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f2b25be elementor-widget elementor-widget-image\" data-id=\"f2b25be\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-09.50.47.png\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e6f7e5a elementor-widget elementor-widget-text-editor\" data-id=\"e6f7e5a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong><em>Figure 2 : Les exosomes sont tr\u00e8s variables en termes de taille, de contenu, de fonctions et de source. Extrait de [1].<\/em><\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-618e19f elementor-widget elementor-widget-text-editor\" data-id=\"618e19f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #800080;\"><strong>Actuellement, 9 769 prot\u00e9ines, 2 838 miRNA, 3 408 mRNA et 1 116 lipides ont \u00e9t\u00e9 d\u00e9crits dans leur composition [3].\u00a0<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Les prot\u00e9ines exosomales sont diff\u00e9rentes selon la nature des cellules ou des tissus primitifs [4].\u00a0<\/span><\/p><p><strong><span style=\"color: #800080;\">Les principales prot\u00e9ines exosomales sont des prot\u00e9ines:<\/span><\/strong><\/p><ul><li><span style=\"color: #800080;\"> de transport et de fusion membranaire, <\/span><\/li><li><span style=\"color: #800080;\">des chaperons, <\/span><\/li><li><span style=\"color: #800080;\">des mol\u00e9cules d&rsquo;adh\u00e9sion, <\/span><\/li><li><span style=\"color: #800080;\">des CMH,<\/span><\/li><li><span style=\"color: #800080;\"> des prot\u00e9ines du cytosquelette <\/span><\/li><li><span style=\"color: #800080;\">et des prot\u00e9ines li\u00e9es aux lipides [5].\u00a0<\/span><\/li><\/ul><p><span style=\"color: #800080;\"><strong>Les cellules produisent des exosomes sp\u00e9cifiques ayant des caract\u00e9ristiques et un contenu, uniques.\u00a0<\/strong><\/span><\/p><ul><li><span style=\"color: #800080;\">Les exosomes provenant de cellules canc\u00e9reuses sont susceptibles d&rsquo;aggraver le cancer, <\/span><\/li><li><span style=\"color: #800080;\">tandis que les exosomes produits par des cellules souches sont susceptibles de promouvoir une activit\u00e9 anti-inflammatoire et r\u00e9g\u00e9n\u00e9ratrice comme leur cellule d&rsquo;origine [5].\u00a0<\/span><\/li><\/ul><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1425f4c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1425f4c\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f387c31\" data-id=\"f387c31\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5c1e837 elementor-widget elementor-widget-heading\" data-id=\"5c1e837\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">M\u00e9thodes d'isolement des exosomes<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1826a36 elementor-widget elementor-widget-text-editor\" data-id=\"1826a36\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #800080;\">Les v\u00e9sicules extracellulaires, que l&rsquo;on trouve dans tous les fluides biologiques, constituent une famille qui comprend:<\/span><\/p><ul><li><span style=\"color: #800080;\"> les exosomes (40 nm \u00e0 160 nm) provenant des endosomes\/corps multiv\u00e9siculaires <\/span><\/li><li><span style=\"color: #000000;\"><span style=\"color: #800080;\">et les microv\u00e9sicules (150 nm \u00e0 1000 nm) provenant de la membrane plasmique [6].<\/span>\u00a0<\/span><\/li><\/ul><p><span style=\"color: #000000;\">Les exosomes sont d\u00e9sormais consid\u00e9r\u00e9s par de nombreuses soci\u00e9t\u00e9s de biotechnologie comme des vecteurs \u00e9mergents [7].\u00a0<\/span><\/p><p><span style=\"color: #800080;\"><strong>Un large \u00e9ventail de m\u00e9thodes d&rsquo;isolement des exosomes \u00e0 partir de fluides biologiques a \u00e9t\u00e9 d\u00e9velopp\u00e9 [8].<\/strong><\/span><\/p><p><span style=\"color: #800080;\">Les exosomes sont isol\u00e9s en fonction de leur taille, de leur densit\u00e9 et de leur immunoaffinit\u00e9 par les m\u00e9thodes suivantes : <\/span><\/p><ul><li><span style=\"color: #800080;\">centrifugation, <\/span><\/li><li><span style=\"color: #800080;\">chromatographie, <\/span><\/li><li><span style=\"color: #800080;\">filtration, <\/span><\/li><li><span style=\"color: #800080;\">pr\u00e9cipitation \u00e0 base de polym\u00e8res <\/span><\/li><li><span style=\"color: #000000;\"><span style=\"color: #800080;\">et s\u00e9paration immunologique (voir tableau 1) [9]<\/span>.\u00a0<\/span><\/li><\/ul><p><span style=\"color: #800080;\"><strong>\u00a0<\/strong><\/span><\/p><p><span style=\"color: #800080;\"><strong>L&rsquo;ultracentrifugation est d\u00e9sormais consid\u00e9r\u00e9e comme l&rsquo;\u00e9talon-or et de nombreux groupes de recherche utilisent cette technique [10].\u00a0<\/strong><\/span><br \/><span style=\"color: #000000;\">Cependant, l&rsquo;ultracentrifugation pr\u00e9sente plusieurs inconv\u00e9nients, notamment la n\u00e9cessit\u00e9 de disposer d&rsquo;un grand volume de fluides biologiques, des temps de traitement longs et une faible reproductibilit\u00e9 [11].\u00a0<\/span><\/p><p><span style=\"color: #800080;\"><strong>Les m\u00e9thodes de production, d&rsquo;isolement et de purification des exosomes ont des effets importants sur le rendement, la viabilit\u00e9 et la fonction (tableau 1).<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Les exosomes provenant de diff\u00e9rents sp\u00e9cimens peuvent avoir des contenus prot\u00e9iques\/lipidiques et luminaux diff\u00e9rents et des caract\u00e9ristiques de s\u00e9dimentation diff\u00e9rentes. <\/span><\/p><ul><li><span style=\"color: #000000;\">Par exemple, les exosomes du tissu adipeux ont une teneur \u00e9lev\u00e9e en lipides et n\u00e9cessitent une adaptation de leurs m\u00e9thodes d&rsquo;isolement [12]. <\/span><\/li><li>Si les exosomes doivent \u00eatre isol\u00e9s \u00e0 partir de milieux de culture, il est tr\u00e8s important d&rsquo;utiliser des milieux sans s\u00e9rum ou du s\u00e9rum bovin f\u0153tal sans exosomes.<\/li><\/ul><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3246c0e elementor-widget elementor-widget-image\" data-id=\"3246c0e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-10.01.22.png\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-73f5057 elementor-widget elementor-widget-text-editor\" data-id=\"73f5057\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong><span style=\"color: #0000ff;\"><em>Tableau 1. M\u00e9thodes d&rsquo;isolement des exosomes (d&rsquo;apr\u00e8s Chesmi et al. 2020 [13]).\u00a0<\/em><\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7e1ecad elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7e1ecad\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ba3ac16\" data-id=\"ba3ac16\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-819ce91 elementor-widget elementor-widget-heading\" data-id=\"819ce91\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\"> Les exosomes dans le diagnostic<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-47ca67c elementor-widget elementor-widget-text-editor\" data-id=\"47ca67c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong><span style=\"color: #800080;\">Les exosomes sont des acteurs cl\u00e9s des communications intracellulaires et ont donc la capacit\u00e9 de d\u00e9terminer la progression d&rsquo;une maladie.\u00a0<\/span><\/strong><span style=\"color: #800080;\"><strong>Ils pr\u00e9sentent donc un grand potentiel comme outil de diagnostic et biomarqueurs pour de nombreuses maladies [2].<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Le contenu des exosomes est modifi\u00e9 au cours d&rsquo;une maladie active, qu&rsquo;il s&rsquo;agisse <\/span><\/p><ul><li><span style=\"color: #000000;\">d&rsquo;un cancer, <\/span><\/li><li><span style=\"color: #000000;\">d&rsquo;une maladie cardiovasculaire, <\/span><\/li><li><span style=\"color: #000000;\">de maladies immunologiques, <\/span><\/li><li><span style=\"color: #000000;\">d&rsquo;infections ou de pathologies du SNC (Alzheimer et Parkinson).<\/span><\/li><li><span style=\"color: #000000;\">Plus un processus inflammatoire est en cours, plus les cellules exsudent des exosomes [14].\u00a0<\/span><\/li><\/ul><p>\u00a0<\/p><p><span style=\"color: #800080;\"><strong>La surveillance des exosomes s\u00e9cr\u00e9t\u00e9s par les cellules canc\u00e9reuses peut se faire par biopsie liquide, car tous les fluides corporels contiennent des exosomes lib\u00e9r\u00e9s par le cancer [15].<\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\">Les exosomes peuvent prot\u00e9ger la d\u00e9gradation rapide des acides nucl\u00e9iques et restent tr\u00e8s stables dans le plasma [16].<\/span><\/li><li><span style=\"color: #000000;\">Des preuves croissantes montrent que cette technique peut \u00eatre utilis\u00e9e pour pr\u00e9dire l&rsquo;existence d&rsquo;une tumeur ainsi que l&rsquo;\u00e9tat d&rsquo;invasion et de m\u00e9tastase. <\/span><\/li><li><span style=\"color: #000000;\">Les marqueurs membranaires exosomaux peuvent \u00eatre utilis\u00e9s pour r\u00e9aliser un sous-typage des exosomes afin de suivre la cellule d&rsquo;origine.\u00a0<\/span><\/li><\/ul><p>\u00a0<\/p><p><span style=\"color: #800080;\"><strong>Le march\u00e9 du diagnostic des exosomes est en phase de d\u00e9veloppement pr\u00e9coce. <\/strong><\/span><span style=\"color: #800080;\"><strong>Des progr\u00e8s techniques et une meilleure compr\u00e9hension de la biologie des exosomes doivent \u00eatre r\u00e9alis\u00e9s avant que cette technique puisse \u00eatre utilis\u00e9e dans les tests diagnostiques de routine.<\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-afcfd72 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"afcfd72\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7471369\" data-id=\"7471369\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5e5fdd3 elementor-widget elementor-widget-heading\" data-id=\"5e5fdd3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Les exosomes en tant que produits th\u00e9rapeutiques<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7bc80be elementor-widget elementor-widget-text-editor\" data-id=\"7bc80be\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong>Les exosomes sont de puissants communicateurs dans l&rsquo;organisme.\u00a0Certains sugg\u00e8rent que presque toutes les activit\u00e9s des cellules souches sont le r\u00e9sultat des exosomes qu&rsquo;elles produisent [17]. De nombreuses \u00e9tudes en cours visent \u00e0 d\u00e9crypter le r\u00f4le des exosomes dans la r\u00e9g\u00e9n\u00e9ration des tissus [5].<\/strong><\/span><\/p><p><span style=\"color: #800080;\"><strong> R\u00e9cemment, l&rsquo;utilisation des cellules souches endog\u00e8nes pour r\u00e9parer les l\u00e9sions tissulaires a suscit\u00e9 un int\u00e9r\u00eat croissant.\u00a0<\/strong><\/span><\/p><ul><li><strong><span style=\"color: #800080;\">Les cellules souches m\u00e9senchymateuses (CSM) ont \u00e9t\u00e9 extr\u00eamement \u00e9tudi\u00e9es, en raison de leur potentiel de diff\u00e9renciation, de leurs effets immunosuppresseurs et de leur relative facilit\u00e9 de culture. <\/span><\/strong><\/li><li><strong><span style=\"color: #800080;\">Ces effets sont connus sous le nom d&rsquo;activit\u00e9 paracrine des CSM, qui contribue \u00e0 leur m\u00e9diation [18].\u00a0<\/span><\/strong><\/li><li><span style=\"color: #800080;\"><strong>De nombreuses \u00e9tudes in vivo ont d\u00e9montr\u00e9 les effets th\u00e9rapeutiques des exosomes d\u00e9riv\u00e9s des CSM [19].\u00a0<\/strong><\/span><\/li><li><span style=\"color: #800080;\"><strong>Trois principaux tissus sont pris en compte lors du pr\u00e9l\u00e8vement de cellules souches ayant un potentiel MSC : la moelle osseuse, le tissu adipeux et le placenta.<\/strong><\/span><\/li><\/ul><p><span style=\"color: #800080;\"><strong><em>Les exosomes d\u00e9riv\u00e9s des CSM peuvent agir comme un suivi dans la cicatrisation des plaies cutan\u00e9es (Figure 3) :<\/em><\/strong><\/span><\/p><ol><li><span style=\"color: #800080;\">R\u00e9duction de l&rsquo;inflammation (due \u00e0 une r\u00e9duction des cellules T pro-inflammatoires et \u00e0 une augmentation du nombre de cellules anti-inflammatoires (cellules T et macrophages) [20] ;<\/span><\/li><li><span style=\"color: #800080;\">Une action r\u00e9g\u00e9n\u00e9ratrice mise en \u00e9vidence par la prolif\u00e9ration des fibroblastes et des k\u00e9ratinocytes au niveau de la plaie [21] ;<\/span><\/li><li><span style=\"color: #800080;\">Une r\u00e9duction de la mort cellulaire et une activit\u00e9 anti-apoptotique [22] ;<\/span><\/li><li><span style=\"color: #800080;\">R\u00e9gulation de l&rsquo;angiogen\u00e8se par activation directe des cellules endoth\u00e9liales [23].<\/span><\/li><\/ol><p><span style=\"color: #000000;\">Une revue syst\u00e9matique a \u00e9valu\u00e9 plus de 200 \u00e9tudes avec des exosomes d\u00e9riv\u00e9s de CSM, d\u00e9montrant des b\u00e9n\u00e9fices dans 72% des \u00e9tudes [24].<\/span><\/p><p><span style=\"color: #800080;\"><strong><em>Les exosomes pr\u00e9sentent diff\u00e9rents avantages par rapport \u00e0 la th\u00e9rapie cellulaire :<\/em><\/strong><\/span><\/p><p><span style=\"color: #800080;\">&#8211; les exosomes sont plus stables et stockables [5] ;<\/span><\/p><p><span style=\"color: #800080;\">&#8211; les exosomes peuvent \u00eatre charg\u00e9s d&rsquo;agents th\u00e9rapeutiques ayant une action cibl\u00e9e sur le site d&rsquo;int\u00e9r\u00eat [25] ;<\/span><\/p><p><span style=\"color: #800080;\">&#8211; les exosomes d\u00e9riv\u00e9s de cellules souches sont moins immunog\u00e8nes que les th\u00e9rapies cellulaires [26] ;<\/span><\/p><p><span style=\"color: #800080;\">&#8211; la th\u00e9rapie par cellules souches n\u00e9cessite une technologie de traitement d\u00e9licate ; les exosomes ne sont pas des cellules et peuvent \u00eatre congel\u00e9s ou lyophilis\u00e9s sans perte d&rsquo;activit\u00e9 biochimique [27] ;<\/span><\/p><p><span style=\"color: #800080;\">&#8211; les cellules souches peuvent subir une transformation n\u00e9oplasique alors que les exosomes n&rsquo;ont pas cette capacit\u00e9 [28].<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a7d7ea7 elementor-widget elementor-widget-image\" data-id=\"a7d7ea7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-10.36.23.png\" title=\"\" alt=\"Biological activities of stem cell derived exosomes on skin wound healing. From Lv et al [29].\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f8d1676 elementor-widget elementor-widget-text-editor\" data-id=\"f8d1676\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong><em>Figure 3.\u00a0Activit\u00e9s biologiques des exosomes d\u00e9riv\u00e9s de cellules souches sur la cicatrisation des plaies cutan\u00e9es. D&rsquo;apr\u00e8s Lv et al [29].\u00a0<\/em><\/strong><\/span><\/p><ol><li><span style=\"color: #000000;\"><em> Les exosomes peuvent limiter l&rsquo;inflammation en agissant sur les cellules inflammatoires et les macrophages.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em> Les exosomes peuvent d\u00e9clencher la r\u00e9\u00e9pith\u00e9lialisation en augmentant les activit\u00e9s des fibroblastes et des k\u00e9ratinocytes.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em> Les exosomes peuvent acc\u00e9l\u00e9rer l&rsquo;angiogen\u00e8se par l&rsquo;activation directe des cellules endoth\u00e9liales.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em> Les exosomes peuvent moduler le remodelage tissulaire via la production de collag\u00e8ne et la diff\u00e9renciation des myofibroblastes.<\/em><\/span><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2244b90 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2244b90\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0f658c6\" data-id=\"0f658c6\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cda52bc elementor-widget elementor-widget-heading\" data-id=\"cda52bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Exigences techniques et r\u00e9glementaires pour une utilisation clinique des exosomes<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-43bdb24 elementor-widget elementor-widget-text-editor\" data-id=\"43bdb24\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #000000;\"><em><strong><span style=\"color: #800080;\">Th\u00e9oriquement, en tant que th\u00e9rapie immunocompatible sans cellules, la technologie des exosomes devrait offrir des avantages r\u00e9glementaires par rapport aux th\u00e9rapies \u00e0 base de cellules souches en termes de processus et de fabrication<\/span><\/strong>.\u00a0<\/em><\/span><span style=\"color: #800080;\"><strong><em>Cependant, \u00e0 ce jour, aucun produit \u00e0 base d&rsquo;exosomes n&rsquo;est actuellement approuv\u00e9.\u00a0<\/em><\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\"><em>La FDA et d&rsquo;autres autorit\u00e9s r\u00e9glementaires dans le monde exigent des contr\u00f4les importants pour la qualit\u00e9, la puret\u00e9, la puissance et la reproductibilit\u00e9.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>Le protocole d&rsquo;optimisation de la m\u00e9thode de purification n&rsquo;est pas trivial alors que les exosomes contiennent une quantit\u00e9 inconnue d&rsquo;exosomes, et sont \u00e9galement contamin\u00e9s par des prot\u00e9ines, de l&rsquo;ADN libre de cellules, des v\u00e9sicules, potentiellement des virus et d&rsquo;autres composants de la m\u00eame gamme de taille.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>Les exosomes peuvent s&rsquo;accumuler dans divers organes et traverser la barri\u00e8re h\u00e9mato-enc\u00e9phalique [30].<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>En outre, les exosomes peuvent perdre ou gagner des fonctions au cours du processus d&rsquo;isolement, ce qui peut \u00eatre potentiellement dangereux pour le patient.\u00a0<\/em><\/span><\/li><\/ul><p><span style=\"color: #800080;\"><strong><em>La th\u00e9rapie par cellules souches m\u00e9senchymateuses (CSM) a suscit\u00e9 l&rsquo;int\u00e9r\u00eat des scientifiques apr\u00e8s la d\u00e9couverte de son potentiel th\u00e9rapeutique. Cependant, leur utilisation clinique a \u00e9t\u00e9 entrav\u00e9e en raison de leur immunog\u00e9nicit\u00e9 et de leur tumorig\u00e9nicit\u00e9.\u00a0<\/em><\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\"><em>Relativement r\u00e9cemment, il a \u00e9t\u00e9 r\u00e9v\u00e9l\u00e9 que le m\u00e9canisme par lequel les MSC favorisent la gu\u00e9rison est la s\u00e9cr\u00e9tion d&rsquo;exosomes.\u00a0<\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>Cela a suscit\u00e9 l&rsquo;int\u00e9r\u00eat pour le d\u00e9veloppement d&rsquo;une th\u00e9rapie sans cellules, \u00e9vitant ainsi les obstacles qui ont emp\u00each\u00e9 l&rsquo;application de la th\u00e9rapie MSC dans la pratique clinique.\u00a0<\/em><\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-88c949f elementor-widget elementor-widget-image\" data-id=\"88c949f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-10.41.48.png\" title=\"\" alt=\"Figure 4: Biological and technical limitations for the use of exosomes in clinic. From [26].\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ab37711 elementor-widget elementor-widget-text-editor\" data-id=\"ab37711\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong>Figure 4: Biological and technical limitations for the use of exosomes in clinic. From <\/strong><strong>[<a style=\"color: #0000ff;\" href=\"#_ENREF_26\">26<\/a><\/strong><strong>]<\/strong><strong>.<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Origine of exosomes affects its biological features (immunity, toxicity, and therapeutic properties) as they are the same as the parental cells.<\/span><\/p><ul><li><span style=\"color: #000000;\"> Isolation procedure (i.e centrifugation) may damage the exosome membrane that may be damagable to patients and are difficult to predict.<\/span><\/li><li><span style=\"color: #000000;\">Environmental culture conditions is affecting the quality and quantity of exosomes production. Injection technique is directly affecting the exosome biodistribution and pharmacokinetics.<\/span><\/li><li><span style=\"color: #000000;\">Intravenous injection bears the risk of exosome phagocytosis but led to a better organ accumulation than after intramuscular or subcutaneous injection.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d353e0a elementor-widget elementor-widget-heading\" data-id=\"d353e0a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Status of fundamental research<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5654880 elementor-widget elementor-widget-text-editor\" data-id=\"5654880\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #800080;\"><strong>Up to now, efficacy and safety are not already been established in human trials. <\/strong><strong>Exosomes in clinical trials need to comply with good manufacturing practice (GMP). <\/strong><\/span><\/p><p><span style=\"color: #000000;\"><strong><span style=\"color: #800080;\">Three important issues are prevalent in GMP for exosomes, i.e., upstream of cell cultivation process, downstream of the purification process, and exosome quality control [<a style=\"color: #800080;\" href=\"#_ENREF_8\">8<\/a>]<\/span>. <\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\">Kwon and colleagues were the first to propose exosomes as a clinical tool with presenting human clinical data in atrophic acne scars [<a style=\"color: #000000;\" href=\"#_ENREF_31\"><strong>31<\/strong><\/a><strong>]<\/strong>.<\/span><\/li><li><span style=\"color: #800080;\"><strong>MSC-derived exosomes were topically applied after carbon dioxide laser treatment.<\/strong> <\/span><span style=\"color: #000000;\">No adverse effects were associated with exosome application. <\/span><span style=\"color: #000000;\">The treated patients displayed sooner and better improvement in atrophic acne scarring. <\/span><span style=\"color: #000000;\">MSC-derived exosomes used were hypo immunogenic and negative for human leukocyte antigen.<\/span><\/li><li><span style=\"color: #000000;\">Early preclinical studies in aesthetics have demonstrated promising effects of exosomes on wound healing for skin rejuvenation and hair growth in <em>in vitro<\/em> and murine models (<strong>Table 2 and Table 3). <\/strong><\/span><\/li><li><span style=\"color: #000000;\">Despite this, only 1 clinical study has been published to date, and there are no FDA-approved products on the market <strong>[<\/strong><a style=\"color: #000000;\" href=\"#_ENREF_32\"><strong>32<\/strong><\/a><strong>]<\/strong><strong>.<\/strong><\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7226ba6 elementor-widget elementor-widget-image\" data-id=\"7226ba6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-10.51.59.png\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b50737f elementor-widget elementor-widget-image\" data-id=\"b50737f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-11-a\u0300-10.52.33.png\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-81eb374 elementor-widget elementor-widget-heading\" data-id=\"81eb374\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Conclusion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d6923e5 elementor-widget elementor-widget-text-editor\" data-id=\"d6923e5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #800080;\"><strong><em>De nombreux essais cliniques ont \u00e9tudi\u00e9 les cellules souches et leur milieu conditionn\u00e9 (MC) \u00e0 des fins esth\u00e9tiques [31,37-42].\u00a0<\/em><\/strong><\/span><\/p><p><span style=\"color: #000000;\"><em>Le milieu conditionn\u00e9 des cellules souches est int\u00e9ressant pour les \u00e9tudes pr\u00e9cliniques car il contient des m\u00e9diateurs paracrines, notamment des exosomes, qui exercent des activit\u00e9s r\u00e9g\u00e9n\u00e9ratrices similaires \u00e0 celles des cellules souches elles-m\u00eames.\u00a0<\/em><\/span><\/p><p><strong><span style=\"color: #800080;\"><em>Il s&rsquo;agit d&rsquo;une alternative libre int\u00e9ressante \u00e0 la th\u00e9rapie par cellules souches.<\/em><\/span><\/strong><\/p><ul><li><span style=\"color: #000000;\"><em>Dans le domaine de l&rsquo;esth\u00e9tique, les exosomes utilis\u00e9s sont le plus souvent d\u00e9riv\u00e9s de MSC adipeuses [43]. <\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>Des exosomes purifi\u00e9s ont \u00e9t\u00e9 utilis\u00e9s avec succ\u00e8s dans des \u00e9tudes pr\u00e9cliniques, mais les essais cliniques en esth\u00e9tique font d\u00e9faut [32]. <\/em><\/span><\/li><li><span style=\"color: #000000;\"><em>D&rsquo;autres \u00e9tudes animales et humaines sur la s\u00e9curit\u00e9 et l&rsquo;efficacit\u00e9 des exosomes sont n\u00e9cessaires avant que la th\u00e9rapie \u00e0 base d&rsquo;exosomes ne devienne une r\u00e9alit\u00e9.<\/em><\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1619a37 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1619a37\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d95bc51\" data-id=\"d95bc51\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d3e0d43 elementor-widget elementor-widget-heading\" data-id=\"d3e0d43\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">R\u00e9f\u00e9rences<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7f19ac7 elementor-widget elementor-widget-text-editor\" data-id=\"7f19ac7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0<\/p><ol><li><span style=\"color: #800080;\">Kalluri, R.; LeBleu, V.S. The biology, function, and biomedical applications of exosomes.\u00a0<em>Science\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>367<\/em>, doi:10.1126\/science.aau6977.<\/span><\/li><li><span style=\"color: #800080;\">Muthu, S.; Bapat, A.; Jain, R.; Jeyaraman, N.; Jeyaraman, M. Exosomal therapy-a new frontier in regenerative medicine.\u00a0<em>Stem cell investigation\u00a0<\/em><strong>2021<\/strong>,\u00a0<em>8<\/em>, 7, doi:10.21037\/sci-2020-037.<\/span><\/li><li><span style=\"color: #800080;\">Mathivanan, S.; Fahner, C.J.; Reid, G.E.; Simpson, R.J. ExoCarta 2012: database of exosomal proteins, RNA and lipids.\u00a0<em>Nucleic acids research\u00a0<\/em><strong>2012<\/strong>,\u00a0<em>40<\/em>, D1241-1244, doi:10.1093\/nar\/gkr828.<\/span><\/li><li><span style=\"color: #800080;\">Mathivanan, S.; Lim, J.W.; Tauro, B.J.; Ji, H.; Moritz, R.L.; Simpson, R.J. Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature.\u00a0<em>Molecular &amp; cellular proteomics : MCP\u00a0<\/em><strong>2010<\/strong>,\u00a0<em>9<\/em>, 197-208, doi:10.1074\/mcp.M900152-MCP200.<\/span><\/li><li><span style=\"color: #800080;\">Basu, J.; Ludlow, J.W. Exosomes for repair, regeneration and rejuvenation.\u00a0<em>Expert opinion on biological therapy\u00a0<\/em><strong>2016<\/strong>,\u00a0<em>16<\/em>, 489-506, doi:10.1517\/14712598.2016.1131976.<\/span><\/li><li><span style=\"color: #800080;\">Yanez-Mo, M.; Siljander, P.R.; Andreu, Z.; Zavec, A.B.; Borras, F.E.; Buzas, E.I.; Buzas, K.; Casal, E.; Cappello, F.; Carvalho, J.; et al. Biological properties of extracellular vesicles and their physiological functions.\u00a0<em>Journal of extracellular vesicles\u00a0<\/em><strong>2015<\/strong>,\u00a0<em>4<\/em>, 27066, doi:10.3402\/jev.v4.27066.<\/span><\/li><li><span style=\"color: #800080;\">Kumar, D.N.; Chaudhuri, A.; Aqil, F.; Dehari, D.; Munagala, R.; Singh, S.; Gupta, R.C.; Agrawal, A.K. Exosomes as Emerging Drug Delivery and Diagnostic Modality for Breast Cancer: Recent Advances in Isolation and Application.\u00a0<em>Cancers\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>14<\/em>, doi:10.3390\/cancers14061435.<\/span><\/li><li><span style=\"color: #800080;\">Chen, Y.S.; Lin, E.Y.; Chiou, T.W.; Harn, H.J. Exosomes in clinical trial and their production in compliance with good manufacturing practice.\u00a0<em>Ci ji yi xue za zhi = Tzu-chi medical journal\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>32<\/em>, 113-120, doi:10.4103\/tcmj.tcmj_182_19.<\/span><\/li><li><span style=\"color: #800080;\">Liu, W.Z.; Ma, Z.J.; Kang, X.W. Current status and outlook of advances in exosome isolation.\u00a0<em>Analytical and bioanalytical chemistry\u00a0<\/em><strong>2022<\/strong>, doi:10.1007\/s00216-022-04253-7.<\/span><\/li><li><span style=\"color: #800080;\">Huang, J.; Xiong, J.; Yang, L.; Zhang, J.; Sun, S.; Liang, Y. Cell-free exosome-laden scaffolds for tissue repair.\u00a0<em>Nanoscale\u00a0<\/em><strong>2021<\/strong>,\u00a0<em>13<\/em>, 8740-8750, doi:10.1039\/d1nr01314a.<\/span><\/li><li><span style=\"color: #800080;\">Wu, X.; Showiheen, S.A.A.; Sun, A.R.; Crawford, R.; Xiao, Y.; Mao, X.; Prasadam, I. Exosomes Extraction and Identification.\u00a0<em>Methods in molecular biology\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>2054<\/em>, 81-91, doi:10.1007\/978-1-4939-9769-5_4.<\/span><\/li><li><span style=\"color: #800080;\">An, Y.; Lin, S.; Tan, X.; Zhu, S.; Nie, F.; Zhen, Y.; Gu, L.; Zhang, C.; Wang, B.; Wei, W.; et al.\u00a0Exosomes from adipose-derived stem cells and application to skin wound healing.\u00a0<em>Cell proliferation\u00a0<\/em><strong>2021<\/strong>,\u00a0<em>54<\/em>, e12993, doi:10.1111\/cpr.12993.<\/span><\/li><li><span style=\"color: #800080;\">Cheshmi, B.; Cheshomi, H. Salivary exosomes: properties, medical applications, and isolation methods.\u00a0<em>Molecular biology reports\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>47<\/em>, 6295-6307, doi:10.1007\/s11033-020-05659-1.<\/span><\/li><li><span style=\"color: #800080;\">Tian, Y.; Cheng, C.; Wei, Y.; Yang, F.; Li, G. The Role of Exosomes in Inflammatory Diseases and Tumor-Related Inflammation.\u00a0<em>Cells\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>11<\/em>, doi:10.3390\/cells11061005.<\/span><\/li><li><span style=\"color: #800080;\">Gao, Z.; Pang, B.; Li, J.; Gao, N.; Fan, T.; Li, Y. Emerging Role of Exosomes in Liquid Biopsy for Monitoring Prostate Cancer Invasion and Metastasis.\u00a0<em>Frontiers in cell and developmental biology\u00a0<\/em><strong>2021<\/strong>,\u00a0<em>9<\/em>, 679527, doi:10.3389\/fcell.2021.679527.<\/span><\/li><li><span style=\"color: #800080;\">Zhou, X.; Xie, F.; Wang, L.; Zhang, L.; Zhang, S.; Fang, M.; Zhou, F. The function and clinical application of extracellular vesicles in innate immune regulation.\u00a0<em>Cellular &amp; molecular immunology\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>17<\/em>, 323-334, doi:10.1038\/s41423-020-0391-1.<\/span><\/li><li><span style=\"color: #800080;\">Allan, D.; Tieu, A.; Lalu, M.; Burger, D. Mesenchymal stromal cell-derived extracellular vesicles for regenerative therapy and immune modulation: Progress and challenges toward clinical application.\u00a0<em>Stem cells translational medicine\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>9<\/em>, 39-46, doi:10.1002\/sctm.19-0114.<\/span><\/li><li><span style=\"color: #800080;\">Xia, J.; Minamino, S.; Kuwabara, K.; Arai, S. Stem cell secretome as a new booster for regenerative medicine.\u00a0<em>Bioscience trends\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>13<\/em>, 299-307, doi:10.5582\/bst.2019.01226.<\/span><\/li><li><span style=\"color: #800080;\">Ha, D.H.; Kim, H.K.; Lee, J.; Kwon, H.H.; Park, G.H.; Yang, S.H.; Jung, J.Y.; Choi, H.; Lee, J.H.; Sung, S.; et al. Mesenchymal Stem\/Stromal Cell-Derived Exosomes for Immunomodulatory Therapeutics and Skin Regeneration.\u00a0<em>Cells\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>9<\/em>, doi:10.3390\/cells9051157.<\/span><\/li><li><span style=\"color: #800080;\">Zhang, B.; Yin, Y.; Lai, R.C.; Tan, S.S.; Choo, A.B.; Lim, S.K. Mesenchymal stem cells secrete immunologically active exosomes.\u00a0<em>Stem cells and development\u00a0<\/em><strong>2014<\/strong>,\u00a0<em>23<\/em>, 1233-1244, doi:10.1089\/scd.2013.0479.<\/span><\/li><li><span style=\"color: #800080;\">Bian, D.; Wu, Y.; Song, G.; Azizi, R.; Zamani, A. The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: a comprehensive review.\u00a0<em>Stem cell research &amp; therapy\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>13<\/em>, 24, doi:10.1186\/s13287-021-02697-9.<\/span><\/li><li><span style=\"color: #800080;\">Zhang, B.; Wang, M.; Gong, A.; Zhang, X.; Wu, X.; Zhu, Y.; Shi, H.; Wu, L.; Zhu, W.; Qian, H.; et al. HucMSC-Exosome Mediated-Wnt4 Signaling Is Required for Cutaneous Wound Healing.\u00a0<em>Stem Cells\u00a0<\/em><strong>2015<\/strong>,\u00a0<em>33<\/em>, 2158-2168, doi:10.1002\/stem.1771.<\/span><\/li><li><span style=\"color: #800080;\">Chen, J.; Liu, Z.; Hong, M.M.; Zhang, H.; Chen, C.; Xiao, M.; Wang, J.; Yao, F.; Ba, M.; Liu, J.; et al. Proangiogenic compositions of microvesicles derived from human umbilical cord mesenchymal stem cells.\u00a0<em>PloS one\u00a0<\/em><strong>2014<\/strong>,\u00a0<em>9<\/em>, e115316, doi:10.1371\/journal.pone.0115316.<\/span><\/li><li><span style=\"color: #800080;\">Tieu, A.; Lalu, M.M.; Slobodian, M.; Gnyra, C.; Fergusson, D.A.; Montroy, J.; Burger, D.; Stewart, D.J.; Allan, D.S. An Analysis of Mesenchymal Stem Cell-Derived Extracellular Vesicles for Preclinical Use.\u00a0<em>ACS nano\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>14<\/em>, 9728-9743, doi:10.1021\/acsnano.0c01363.<\/span><\/li><li><span style=\"color: #800080;\">Bunggulawa, E.J.; Wang, W.; Yin, T.; Wang, N.; Durkan, C.; Wang, Y.; Wang, G. Recent advancements in the use of exosomes as drug delivery systems.\u00a0<em>Journal of nanobiotechnology\u00a0<\/em><strong>2018<\/strong>,\u00a0<em>16<\/em>, 81, doi:10.1186\/s12951-018-0403-9.<\/span><\/li><li><span style=\"color: #800080;\">Szwedowicz, U.; Lapinska, Z.; Gajewska-Naryniecka, A.; Choromanska, A. Exosomes and Other Extracellular Vesicles with High Therapeutic Potential: Their Applications in Oncology, Neurology, and Dermatology.\u00a0<em>Molecules\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>27<\/em>, doi:10.3390\/molecules27041303.<\/span><\/li><li><span style=\"color: #800080;\">Kao, C.Y.; Papoutsakis, E.T. Extracellular vesicles: exosomes, microparticles, their parts, and their targets to enable their biomanufacturing and clinical applications.\u00a0<em>Current opinion in biotechnology\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>60<\/em>, 89-98, doi:10.1016\/j.copbio.2019.01.005.<\/span><\/li><li><span style=\"color: #800080;\">Carson, C.T.; Aigner, S.; Gage, F.H. Stem cells: the good, bad and barely in control.\u00a0<em>Nature medicine\u00a0<\/em><strong>2006<\/strong>,\u00a0<em>12<\/em>, 1237-1238, doi:10.1038\/nm1106-1237.<\/span><\/li><li><span style=\"color: #800080;\">Lv, H.; Liu, H.; Sun, T.; Wang, H.; Zhang, X.; Xu, W. Exosome derived from stem cell: A promising therapeutics for wound healing.\u00a0<em>Frontiers in pharmacology\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>13<\/em>, 957771, doi:10.3389\/fphar.2022.957771.<\/span><\/li><li><span style=\"color: #800080;\">D&rsquo;Anca, M.; Fenoglio, C.; Serpente, M.; Arosio, B.; Cesari, M.; Scarpini, E.A.; Galimberti, D. Exosome Determinants of Physiological Aging and Age-Related Neurodegenerative Diseases.\u00a0<em>Frontiers in aging neuroscience\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>11<\/em>, 232, doi:10.3389\/fnagi.2019.00232.<\/span><\/li><li><span style=\"color: #800080;\">Kwon, H.H.; Yang, S.H.; Lee, J.; Park, B.C.; Park, K.Y.; Jung, J.Y.; Bae, Y.; Park, G.H. Combination Treatment with Human Adipose Tissue Stem Cell-derived Exosomes and Fractional CO2 Laser for Acne Scars: A 12-week Prospective, Double-blind, Randomized, Split-face Study.\u00a0<em>Acta dermato-venereologica\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>100<\/em>, adv00310, doi:10.2340\/00015555-3666.<\/span><\/li><li><span style=\"color: #800080;\">Hartman, N.; Loyal, J.; Fabi, S. Update on Exosomes in Aesthetics.\u00a0<em>Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]\u00a0<\/em><strong>2022<\/strong>,\u00a0<em>48<\/em>, 862-865, doi:10.1097\/DSS.0000000000003487.<\/span><\/li><li><span style=\"color: #800080;\">Hu, L.; Wang, J.; Zhou, X.; Xiong, Z.; Zhao, J.; Yu, R.; Huang, F.; Zhang, H.; Chen, L. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts.\u00a0<em>Scientific reports\u00a0<\/em><strong>2016<\/strong>,\u00a0<em>6<\/em>, 32993, doi:10.1038\/srep32993.<\/span><\/li><li><span style=\"color: #800080;\">Liu, W.; Yu, M.; Xie, D.; Wang, L.; Ye, C.; Zhu, Q.; Liu, F.; Yang, L. Melatonin-stimulated MSC-derived exosomes improve diabetic wound healing through regulating macrophage M1 and M2 polarization by targeting the PTEN\/AKT pathway.\u00a0<em>Stem cell research &amp; therapy\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>11<\/em>, 259, doi:10.1186\/s13287-020-01756-x.<\/span><\/li><li><span style=\"color: #800080;\">Kim, Y.J.; Yoo, S.M.; Park, H.H.; Lim, H.J.; Kim, Y.L.; Lee, S.; Seo, K.W.; Kang, K.S. Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin.\u00a0<em>Biochemical and biophysical research communications\u00a0<\/em><strong>2017<\/strong>,\u00a0<em>493<\/em>, 1102-1108, doi:10.1016\/j.bbrc.2017.09.056.<\/span><\/li><li><span style=\"color: #800080;\">Shafei, S.; Khanmohammadi, M.; Heidari, R.; Ghanbari, H.; Taghdiri Nooshabadi, V.; Farzamfar, S.; Akbariqomi, M.; Sanikhani, N.S.; Absalan, M.; Tavoosidana, G. Exosome loaded alginate hydrogel promotes tissue regeneration in full-thickness skin wounds: An in vivo study.\u00a0<em>Journal of biomedical materials research. Part A\u00a0<\/em><strong>2020<\/strong>,\u00a0<em>108<\/em>, 545-556, doi:10.1002\/jbm.a.36835.<\/span><\/li><li><span style=\"color: #800080;\">Prakoeswa, C.R.S.; Pratiwi, F.D.; Herwanto, N.; Citrashanty, I.; Indramaya, D.M.; Murtiastutik, D.; Sukanto, H.; Rantam, F.A. The effects of amniotic membrane stem cell-conditioned medium on photoaging.\u00a0<em>The Journal of dermatological treatment\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>30<\/em>, 478-482, doi:10.1080\/09546634.2018.1530438.<\/span><\/li><li><span style=\"color: #800080;\">Wang, X.; Shu, X.; Huo, W.; Zou, L.; Li, L. Efficacy of protein extracts from medium of Adipose-derived stem cells via microneedles on Asian skin.\u00a0<em>Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology\u00a0<\/em><strong>2018<\/strong>,\u00a0<em>20<\/em>, 237-244, doi:10.1080\/14764172.2017.1400171.<\/span><\/li><li><span style=\"color: #800080;\">Lee, H.J.; Lee, E.G.; Kang, S.; Sung, J.H.; Chung, H.M.; Kim, D.H. Efficacy of microneedling plus human stem cell conditioned medium for skin rejuvenation: a randomized, controlled, blinded split-face study.\u00a0<em>Annals of dermatology\u00a0<\/em><strong>2014<\/strong>,\u00a0<em>26<\/em>, 584-591, doi:10.5021\/ad.2014.26.5.584.<\/span><\/li><li><span style=\"color: #800080;\">Amirkhani, M.A.; Shoae-Hassani, A.; Soleimani, M.; Hejazi, S.; Ghalichi, L.; Nilforoushzadeh, M.A. Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study.\u00a0<em>BioImpacts : BI\u00a0<\/em><strong>2016<\/strong>,\u00a0<em>6<\/em>, 149-154, doi:10.15171\/bi.2016.21.<\/span><\/li><li><span style=\"color: #800080;\">Seo, K.Y.; Kim, D.H.; Lee, S.E.; Yoon, M.S.; Lee, H.J. Skin rejuvenation by microneedle fractional radiofrequency and a human stem cell conditioned medium in Asian skin: a randomized controlled investigator blinded split-face study.\u00a0<em>Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology\u00a0<\/em><strong>2013<\/strong>,\u00a0<em>15<\/em>, 25-33, doi:10.3109\/14764172.2012.748201.<\/span><\/li><li><span style=\"color: #800080;\">Zhou, B.R.; Zhang, T.; Bin Jameel, A.A.; Xu, Y.; Xu, Y.; Guo, S.L.; Wang, Y.; Permatasari, F.; Luo, D. The efficacy of conditioned media of adipose-derived stem cells combined with ablative carbon dioxide fractional resurfacing for atrophic acne scars and skin rejuvenation.\u00a0<em>Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology\u00a0<\/em><strong>2016<\/strong>,\u00a0<em>18<\/em>, 138-148, doi:10.3109\/14764172.2015.1114638.<\/span><\/li><li><span style=\"color: #800080;\">Li, L.; Ngo, H.T.T.; Hwang, E.; Wei, X.; Liu, Y.; Liu, J.; Yi, T.H. Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts.\u00a0<em>International journal of molecular sciences\u00a0<\/em><strong>2019<\/strong>,\u00a0<em>21<\/em>, doi:10.3390\/ijms21010049.<\/span><\/li><\/ol><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Un autre regard Les exosomes : une innovation en m\u00e9decine esth\u00e9tique Sarah Berndt, PhD Docteur en sciences biom\u00e9dicales et pharmaceutiques Responsable de la recherche en th\u00e9rapie cellulaire chez Regen Lab SA (Suisse) Introduction Les exosomes sont de petites v\u00e9sicules extracellulaires rondes produites par presque tous les types de cellules et pr\u00e9sentes dans tous les fluides [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":13425,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[152],"tags":[299,290],"auteur":[423],"mots-cles":[],"class_list":["post-13537","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-un-autre-regards","tag-lm3-un-autre-regard","tag-replay-lm3","auteur-sarah-berndt"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique<\/title>\n<meta name=\"description\" content=\"Le tissu adipeux pr\u00e9sente l\u2019avantage de la facilit\u00e9 de pr\u00e9l\u00e8vement et la possibilit\u00e9 de pouvoir r\u00e9aliser des autogreffes.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/13537\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\" \/>\n<meta property=\"og:description\" content=\"Le tissu adipeux pr\u00e9sente l\u2019avantage de la facilit\u00e9 de pr\u00e9l\u00e8vement et la possibilit\u00e9 de pouvoir r\u00e9aliser des autogreffes.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/looketmedecine.fr\/en\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/\" \/>\n<meta property=\"og:site_name\" content=\"Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/looketmedecine.magazine\/\" \/>\n<meta property=\"article:published_time\" content=\"2022-10-11T07:37:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-10-05T20:29:18+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1736\" \/>\n\t<meta property=\"og:image:height\" content=\"1630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Albert-Claude Benhamou\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Albert-Claude Benhamou\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"19 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/\"},\"author\":{\"name\":\"Albert-Claude Benhamou\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#\\\/schema\\\/person\\\/d674c704426e829b385c4dd691b8e3cc\"},\"headline\":\"LM N\u00b0 2\\\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques\",\"datePublished\":\"2022-10-11T07:37:01+00:00\",\"dateModified\":\"2024-10-05T20:29:18+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/\"},\"wordCount\":3848,\"publisher\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2022\\\/10\\\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png\",\"keywords\":[\"Lm3 un autre regard\",\"Replay lm3\"],\"articleSection\":[\"Un autre regard\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/\",\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/\",\"name\":\"LM N\u00b0 2\\\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2022\\\/10\\\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png\",\"datePublished\":\"2022-10-11T07:37:01+00:00\",\"dateModified\":\"2024-10-05T20:29:18+00:00\",\"description\":\"Le tissu adipeux pr\u00e9sente l\u2019avantage de la facilit\u00e9 de pr\u00e9l\u00e8vement et la possibilit\u00e9 de pouvoir r\u00e9aliser des autogreffes.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#primaryimage\",\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2022\\\/10\\\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png\",\"contentUrl\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2022\\\/10\\\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png\",\"width\":1736,\"height\":1630,\"caption\":\"Sarah Berndt. PhD in Biomedical and Pharmaceutical Sciences- Head of Cell Therapy Research at Regen Lab SA\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/looketmedecine.fr\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"LM N\u00b0 2\\\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#website\",\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/\",\"name\":\"Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique\",\"description\":\"La sant\u00e9 esth\u00e9tique fond\u00e9e sur des preuves scientifiques\",\"publisher\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/looketmedecine.fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#organization\",\"name\":\"Looketmedecine\",\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2020\\\/11\\\/cropped-LookMedecine_Logo_Textuel_Noir.png\",\"contentUrl\":\"https:\\\/\\\/looketmedecine.fr\\\/wp-content\\\/uploads\\\/2020\\\/11\\\/cropped-LookMedecine_Logo_Textuel_Noir.png\",\"width\":1500,\"height\":145,\"caption\":\"Looketmedecine\"},\"image\":{\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/looketmedecine.magazine\\\/\",\"https:\\\/\\\/www.instagram.com\\\/looketmedecine\\\/?hl=fr\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/looketmedecine.fr\\\/#\\\/schema\\\/person\\\/d674c704426e829b385c4dd691b8e3cc\",\"name\":\"Albert-Claude Benhamou\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g\",\"caption\":\"Albert-Claude Benhamou\"},\"url\":\"https:\\\/\\\/looketmedecine.fr\\\/en\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","description":"The advantage of adipose tissue is that it is easy to harvest and can be used for autografts.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/13537","og_locale":"en_US","og_type":"article","og_title":"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","og_description":"Le tissu adipeux pr\u00e9sente l\u2019avantage de la facilit\u00e9 de pr\u00e9l\u00e8vement et la possibilit\u00e9 de pouvoir r\u00e9aliser des autogreffes.","og_url":"https:\/\/looketmedecine.fr\/en\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/","og_site_name":"Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","article_publisher":"https:\/\/www.facebook.com\/looketmedecine.magazine\/","article_published_time":"2022-10-11T07:37:01+00:00","article_modified_time":"2024-10-05T20:29:18+00:00","og_image":[{"width":1736,"height":1630,"url":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png","type":"image\/png"}],"author":"Albert-Claude Benhamou","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Albert-Claude Benhamou","Est. reading time":"19 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#article","isPartOf":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/"},"author":{"name":"Albert-Claude Benhamou","@id":"https:\/\/looketmedecine.fr\/#\/schema\/person\/d674c704426e829b385c4dd691b8e3cc"},"headline":"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques","datePublished":"2022-10-11T07:37:01+00:00","dateModified":"2024-10-05T20:29:18+00:00","mainEntityOfPage":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/"},"wordCount":3848,"publisher":{"@id":"https:\/\/looketmedecine.fr\/#organization"},"image":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#primaryimage"},"thumbnailUrl":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png","keywords":["Lm3 un autre regard","Replay lm3"],"articleSection":["Un autre regard"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/","url":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/","name":"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques - Revue fran\u00e7aise N\u00b01 pour la recherche en sant\u00e9 esth\u00e9tique","isPartOf":{"@id":"https:\/\/looketmedecine.fr\/#website"},"primaryImageOfPage":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#primaryimage"},"image":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#primaryimage"},"thumbnailUrl":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png","datePublished":"2022-10-11T07:37:01+00:00","dateModified":"2024-10-05T20:29:18+00:00","description":"The advantage of adipose tissue is that it is easy to harvest and can be used for autografts.","breadcrumb":{"@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#primaryimage","url":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png","contentUrl":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2022\/10\/Capture-de\u0301cran-2022-10-09-a\u0300-17.23.31.png","width":1736,"height":1630,"caption":"Sarah Berndt. PhD in Biomedical and Pharmaceutical Sciences- Head of Cell Therapy Research at Regen Lab SA"},{"@type":"BreadcrumbList","@id":"https:\/\/looketmedecine.fr\/lm3-un-autre-regard-les-exosomes-une-innovation-en-medecine-esthetique-dr-sarah-berndt-docteur-en-sciences-biomedicales-et-pharmaceutiques\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/looketmedecine.fr\/"},{"@type":"ListItem","position":2,"name":"LM N\u00b0 2\/ 2022. Un autre regard. Les exosomes : une innovation en m\u00e9decine esth\u00e9tique. Dr Sarah Berndt Docteur en sciences biom\u00e9dicales et pharmaceutiques"}]},{"@type":"WebSite","@id":"https:\/\/looketmedecine.fr\/#website","url":"https:\/\/looketmedecine.fr\/","name":"N\u00b01 French magazine for research in aesthetic health","description":"Aesthetic health based on scientific evidence","publisher":{"@id":"https:\/\/looketmedecine.fr\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/looketmedecine.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/looketmedecine.fr\/#organization","name":"Looketmedicine","url":"https:\/\/looketmedecine.fr\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/looketmedecine.fr\/#\/schema\/logo\/image\/","url":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2020\/11\/cropped-LookMedecine_Logo_Textuel_Noir.png","contentUrl":"https:\/\/looketmedecine.fr\/wp-content\/uploads\/2020\/11\/cropped-LookMedecine_Logo_Textuel_Noir.png","width":1500,"height":145,"caption":"Looketmedecine"},"image":{"@id":"https:\/\/looketmedecine.fr\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/looketmedecine.magazine\/","https:\/\/www.instagram.com\/looketmedecine\/?hl=fr"]},{"@type":"Person","@id":"https:\/\/looketmedecine.fr\/#\/schema\/person\/d674c704426e829b385c4dd691b8e3cc","name":"Albert-Claude Benhamou","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/f4c13b834cf55e3822893169175e3671a59788fb9e2d84d3a223c96e83a8f09e?s=96&d=mm&r=g","caption":"Albert-Claude Benhamou"},"url":"https:\/\/looketmedecine.fr\/en"}]}},"_links":{"self":[{"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/13537","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/comments?post=13537"}],"version-history":[{"count":64,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/13537\/revisions"}],"predecessor-version":[{"id":23896,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/posts\/13537\/revisions\/23896"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/media\/13425"}],"wp:attachment":[{"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/media?parent=13537"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/categories?post=13537"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/tags?post=13537"},{"taxonomy":"auteur","embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/auteur?post=13537"},{"taxonomy":"mots-cles","embeddable":true,"href":"https:\/\/looketmedecine.fr\/en\/wp-json\/wp\/v2\/mots-cles?post=13537"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}